Heterotrophic Denitrification Of Wastewater By Using Melanoidin As Sole Carbon Source

Israr Ahmed; Naveed Ahmed Qambrani; Sana Saeed

Authors

Israr Ahmed Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.
Naveed Ahmed Qambrani Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.
Sana Saeed Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.

Keywords:

Heterotrophic, Denitrification, Master Culture Reactor, Toxification

Abstract

Removing nitrate effects through heterotrophic denitrification is an effective approach to treating water or wastewater containing these contaminants. The effective management of wastewater is of paramount importance to mitigate its adverse impacts on both human health and the environment. This study delves into the biodegradation and removal of nitrate and nitrite by introducing melanoidin as a carbon source, the research explores the influence of batch test strategy using a heterotrophic denitrification process for removal of toxicity by using inoculum from MCR (master culture reactor) for 48 hours in which four controls (C1, C2, C3, C4) and five test sample of different dilutions of melanoidin i.e. 100 ppm, 250 ppm, 500ppm, 700 ppm and 1000 ppm were used. The results demonstrate that remarkable removal of nitrate, nitrite, and TOC was found in 100 ppm, 250 ppm and 500 ppm dilutions, with a marked reduction of Total organic carbon (TOC), indicating successful toxicity removal, 750 ppm and 1000 ppm dilutions were not affected by denitrification in TOC removal. Subsequent denitrification of the T1, T2, and T3 samples showcases the potent synergy between treatment processes. Through the use of a C:N ratio of 2:1 and 3:1, 98.14% of nitrate was successfully removed within 48 hours. High-Performance Ion Chromatography (HPIC) was employed to analyze the samples treated with denitrification, revealing the complete elimination of toxicity. These results highlight the crucial role of melanoidin as a carbon source in denitrification, enabling thorough nitrate degradation and detoxification. This study underscores the importance of adopting innovative treatment methods to address the growing challenges associated with wastewater management.

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