Heterotrophic Denitrification Of Wastewater By Using Melanoidin As Sole Carbon Source

Israr Ahmed; Naveed Ahmed Qambrani; Sana Saeed

doi:10.33411/IJIST/834

Authors

Israr Ahmed Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.
Naveed Ahmed Qambrani Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.
Sana Saeed Centre of Uspcas-w, Mehran University of science and technology, Jamshoro, Sindh, Pakistan.

DOI:

https://doi.org/10.33411/IJIST/834

Keywords:

Heterotrophic, Denitrification, Master Culture Reactor, Toxification

Abstract

Removal of nitrate through heterotrophic denitrification is a highly effective method for treating water and wastewater containing these contaminants. Proper management of wastewater is critical to mitigating its harmful effects on both human health and theenvironment. This study investigates the biodegradation and removal of nitrate and nitrite by introducing melanoidin as a carbon source, focusing on the impact of a batch test strategy using heterotrophic denitrification. The process employed inoculum from a Master Culture Reactor (MCR) over 48 hours, involving four controls (C1, C2, C3, C4) and five test samples with varying dilutions of melanoidin—100 ppm, 250 ppm, 500 ppm, 700 ppm, and 1000 ppm.The results reveal a significant reduction in nitrate, nitrite, and Total Organic Carbon (TOC) levels, particularly in the 100 ppm, 250 ppm, and 500 ppm melanoidin dilutions, indicating successful toxicity removal. However, the 750 ppm and 1000 ppm dilutions did not show effective TOC removal through denitrification. The subsequent denitrification of samples T1, T2, and T3 demonstrated the potent synergy between treatment processes, with a C ratio of 2:1 and 3:1, leading to 98.14% nitrate removal within 48 hours. High-Performance Ion Chromatography (HPIC) analysis of the denitrified samples confirmed the complete elimination of toxicity.These findings emphasize the critical role of melanoidin as a carbon source in enhancing denitrification, enabling thorough nitrate degradation and detoxification. This study highlights the importance of adopting innovative treatment approaches to address the escalating challenges in wastewater management.

References

Sociol Health Illn.

AZZIZ, G., ILLARZE, G. & IRISARRI, P. 2016. Heterotrophic Denitrification and Paracoccus spp. as Tools for Bioremediation. In: CASTRO-SOWINSKI, S. (ed.) Microbial Models: From Environmental to Industrial Sustainability. Singapore: Springer Singapore.

CHEN, H., YE, Q., WANG, X., SHENG, J., YU, X., ZHAO, S., ZOU, X., ZHANG, W. & XUE, G. 2024. Applying sludge hydrolysate as a carbon source for biological denitrification after composition optimization via red soil filtration. Water research, 249, 120909.

FANG, F., YANG, J., CHEN, L.-L., XU, R.-Z., LUO, J.-Y., NI, B.-J. & CAO, J.-S. 2024. Mixotrophic denitrification of waste activated sludge fermentation liquid as an alternative carbon source for nitrogen removal: Reducing N2O emissions and costs. Journal of Environmental Management, 362, 121348.

FERNÁNDEZ-NAVA, Y., MARANON, E., SOONS, J. & CASTRILLÓN, L. 2008. Denitrification of wastewater containing high nitrate and calcium concentrations. Bioresource Technology, 99, 7976-7981.

FU, X., HOU, R., YANG, P., QIAN, S., FENG, Z., CHEN, Z., WANG, F., YUAN, R., CHEN, H. & ZHOU, B. 2022. Application of external carbon source in heterotrophic denitrification of domestic sewage: A review. Science of The Total Environment, 817, 153061.

GONSALEZ, V. L., DOMBROWSKI, P. M., LEE, M. D. & RAMSBURG, C. A. 2023. The Influence of pH on Subsurface Denitrification Stimulated with Emulsified Vegetable Oil. Water, 15, 883.

MAHMOUD, A., HAMZA, R. A. & ELBESHBISHY, E. 2022. Enhancement of denitrification efficiency using municipal and industrial waste fermentation liquids as external carbon sources. Science of The Total Environment, 816, 151578.

PAINTER, S. C., ARTIOLI, Y., AMIR, F. H., ARNULL, J., GANESHRAM, R. S., IBRAHIM, N., SAMUEL, V. D., ROBIN, R., RAGHURAMAN, R. & PURVAJA, R. 2023. Anthropogenic nitrogen pollution threats and challenges to the health of South Asian coral reefs. Frontiers in Marine Science, 10, 1187804.

PANT, D. & ADHOLEYA, A. 2007. Biological approaches for treatment of distillery wastewater: A review. Bioresource Technology, 98, 2321-2334.

QAMBRANI, N. A., JUNG, S. H., OK, Y. S., KIM, Y. S. & OH, S.-E. 2013. Nitrate-contaminated groundwater remediation by combined autotrophic and heterotrophic denitrification for sulfate and pH control: batch tests. Environmental Science and Pollution Research, 20, 9084-9091.

SCHROEDER, A., SOUZA, D. H., FERNANDES, M., RODRIGUES, E. B., TREVISAN, V. & SKORONSKI, E. 2020. Application of glycerol as carbon source for continuous drinking water denitrification using microorganism from natural biomass. Journal of Environmental Management, 256, 109964.

SINGH, N. & SANTAL, A. 2013. Biodegradation of Melanoidin from Distillery Effluent: Role of Microbes and Their Potential Enzymes.

WANG, H., CHEN, N., FENG, C. & DENG, Y. 2021. Insights into heterotrophic denitrification diversity in wastewater treatment systems: Progress and future prospects based on different carbon sources. Science of The Total Environment, 780, 146521.

ZOU, Y., XU, X., WANG, X., YANG, F. & ZHANG, S. 2018. Achieving efficient nitrogen removal and nutrient recovery from wastewater in a combining simultaneous partial nitrification, anammox and denitrification (SNAD) process with a photobioreactor (PBR) for biomass production and generated dissolved oxygen (DO) recycling. Bioresource Technology, 268, 539-548.