Molecular characterization of Deciphering Fungal Community structure in Zea mays L. and Triticum Aestivum L


  • Kainat Riphah International University Islamabad
  • Waseem Institute of Microbiology and Molecular Genetics. University of the Punjab Lahore
  • Iqra National University of Modern Languages Islamabad
  • Samreen Institute of Microbiology and Molecular Genetics. University of the Punjab Lahore


Fungi , Metagenomics , Pathogen, Nutrients, Rhizosphere


Rhizosphere fungi are strongly associated with plant growth and health by providing nutrients and antagonizing pathogens. Commercially, fungus has multipurpose applications in several sectors including beverages, food items and in medicines. Current study aimed to reveal the core fungal community structure of the two leading cereal crops that are Zea mays L. and Triticum aestivum L. The rhizosphere fungal community was explored via morphology, biochemistry and internal transcribe spacer (ITS) metagenomics. On the basis of morphology, the retrieved fungal strains were imprecisely classified into Ascomycota and Zygomycota. The species including Yeast, Botyritis californica, Rhizopus stolonifer, Alternaria tenuissima, Aspergillus terreus, Aspergillus flavus, Aspergillus nidulans, Aspergillus niger and Microsporum canis were identified on the basis of macroscopy and microscope. Moreover, the biochemical characterization depicted the role of fungi in promotion of plant growth. Majority of the isolates depicted catalase activity, indole production, phosphate solubilization, ammonia production, nitrogenase activity and urease activity. Metagenomics using amplicon sequencing of ITS region revealed the presence of 805 Operational Taxonomic Units (OTUs) with 647 OTUs in Zea mays and 620 OTUs in Triticum aestivum. The fungal phyla found in the rhizosphere of  Zea mays L. and Triticum aestivum L. were Ascomycota, Basidiomycota, Zygomycota, Chytridiomycota, Incertae sedis fungi. Ascomycota accounted for 93% and 95% of classified fungi in rhizosphere of Zea mays L. and Triticum aestivum L. respectively. The dominant species found in the rhizosphere soil of Zea mays were Gibberella intricans, Curvularia lunata, Lepidosphaeria nicotiae, Edenia gomezpompae and Myrothecium verrucaria.


“Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | Home.” (accessed Jun. 21, 2022).

P. R. Ryan, E. Delhaize, and D. L. Jones, “FUNCTION AND MECHANISM OF ORGANIC ANION EXUDATION FROM PLANT ROOTS,” Annu. Rev. Plant Physiol. Plant Mol. Biol., vol. 52, pp. 527–560, 2001, doi: 10.1146/ANNUREV.ARPLANT.52.1.527.

S. Mazhar, R. Yasmeen, A. Chaudhry, and K. Summia, “Role of Microorganisms in Modern Food Industry,” Int. J. Innov. Sci. Technol., vol. 4, no. 1, pp. 65–77, 2022, [Online]. Available:

S. Bokhari, R. Yasmeen, A. W. Qurashi, S. Habib, and U. Rafi, “Isolation of Keratinolytic from Chicken ( Gallus gallus domesticus ) Farms and Assessment of their Efficacy in Feathers Degradation,” Int. J. Innov. Sci. Technol., vol. 3, no. 4, pp. 142–151, 2021, [Online]. Available:

D. L. Jones, C. Nguyen, and R. D. Finlay, “Carbon flow in the rhizosphere: carbon trading at the soil–root interface,” Plant Soil 2009 3211, vol. 321, no. 1, pp. 5–33, Feb. 2009, doi: 10.1007/S11104-009-9925-0.

H. Höper, C. Steinberg, and C. Alabouvette, “Involvement of clay type and pH in the mechanisms of soil suppressiveness to fusarium wilt of flax,” Soil Biol. Biochem., vol. 27, no. 7, pp. 955–967, Jul. 1995, doi: 10.1016/0038-0717(94)00238-V.

M. M. Hossain, F. Sultana, and S. Islam, “Plant growth-promoting fungi (PGPF): Phytostimulation and induced systemic resistance,” Plant-Microbe Interact. Agro-Ecological Perspect., vol. 2, pp. 135–191, Dec. 2017, doi: 10.1007/978-981-10-6593-4_6/COVER/.

N. O. Igiehon and O. O. Babalola, “Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture,” Int. J. Environ. Res. Public Health, vol. 15, no. 4, Apr. 2018, doi: 10.3390/IJERPH15040574.

D. P. Singh, H. B. Singh, and R. Prabha, “Microbial inoculants in sustainable agricultural productivity: Vol. 1: Research perspectives,” Microb. Inoculants Sustain. Agric. Product. Vol. 1 Res. Perspect., pp. 1–343, Jan. 2016, doi: 10.1007/978-81-322-2647-5/COVER.

J. F. Toljander, J. C. Santos-González, A. Tehler, and R. D. Finlay, “Community analysis of arbuscular mycorrhizal fungi and bacteria in the maize mycorrhizosphere in a long-term fertilization trial,” FEMS Microbiol. Ecol., vol. 65, no. 2, pp. 323–338, 2008, doi: 10.1111/j.1574-6941.2008.00512.x.

F. J. Bergersen, “Biochemistry of Symbiotic Nitrogen Fixation in Legumes,”, vol. 22, no. 1, pp. 121–140, Nov. 2003, doi: 10.1146/ANNUREV.PP.22.060171.001005.

A. M. JAROSZ and A. L. DAVELOS, “Effects of disease in wild plant populations and the evolution of pathogen aggressiveness,” New Phytol., vol. 129, no. 3, pp. 371–387, Mar. 1995, doi: 10.1111/J.1469-8137.1995.TB04308.X.

P. A. H. M. Bakker, R. L. Berendsen, R. F. Doornbos, P. C. A. Wintermans, and C. M. J. Pieterse, “The rhizosphere revisited: Root microbiomics,” Front. Plant Sci., vol. 4, no. MAY, p. 165, May 2013, doi: 10.3389/FPLS.2013.00165/BIBTEX.

I. R. Imran, “Environmental Factors as Diabetogenic Agent in Type 2 Diabetes Mellitus,” Int. J. Innov. Sci. Technol., vol. 4, no. April, pp. 288–299, 2022, [Online]. Available:

Z. Hussain, A. Martin, and G. A. Youngberg, “Blastomyces dermatitidis with large yeast forms,” Arch. Pathol. Lab. Med., vol. 125, no. 5, pp. 663–664, 2001, doi: 10.5858/2001-125-0663-BDWLYF.

R. Kaine, Medical microbiology 2003, vol. 53, no. 9. 2003.

B. R. Glick, “Bacteria with ACC deaminase can promote plant growth and help to feed the world,” Microbiol. Res., vol. 169, no. 1, pp. 30–39, Jan. 2014, doi: 10.1016/J.MICRES.2013.09.009.

R. C. Edgar, B. J. Haas, J. C. Clemente, C. Quince, and R. Knight, “UCHIME improves sensitivity and speed of chimera detection,” Bioinformatics, vol. 27, no. 16, pp. 2194–2200, Aug. 2011, doi: 10.1093/BIOINFORMATICS/BTR381.

D. Rotenberg, L. Cooperband, and A. Stone, “Dynamic relationships between soil properties and foliar disease as affected by annual additions of organic amendment to a sandy-soil vegetable production system,” Soil Biol. Biochem., vol. 37, no. 7, pp. 1343–1357, Jul. 2005, doi: 10.1016/J.SOILBIO.2004.12.006.

O. P. Ahlawat, R. Tiwari, and G. P. Singh, “Metagenomics of wheat rhizosphere for abiotic stress management,” Wheat Barley Res., vol. 10, no. 2, 2018, doi: 10.25174/2249-4065/2018/79291.

S. N. Abbas and A. Naushad, “COVID-19 pandemic: A remedial measure through convalescent serum,” Int. J. Innov. Sci. Technol., vol. 2, no. 2, pp. 46–50, 2020.

H. P. Bais, T. L. Weir, L. G. Perry, S. Gilroy, and J. M. Vivanco, “The role of root exudates in rhizosphere interactions with plants and other organisms,” Annu. Rev. Plant Biol., vol. 57, no. February 2006, pp. 233–266, 2006, doi: 10.1146/annurev.arplant.57.032905.105159.

F. A. Ripa, W. D. Cao, S. Tong, and J. G. Sun, “Assessment of plant growth promoting and abiotic stress tolerance properties of wheat endophytic fungi,” Biomed Res. Int., vol. 2019, 2019, doi: 10.1155/2019/6105865.

F. Elias, D. Woyessa, and D. Muleta, “Phosphate Solubilization Potential of Rhizosphere Fungi Isolated from Plants in Jimma Zone, Southwest Ethiopia,” Int. J. Microbiol., vol. 2016, 2016, doi: 10.1155/2016/5472601.

C. Hamel, V. Vujanovic, R. Jeannotte, A. Nakano-Hylander, and M. St-Arnaud, “Negative feedback on a perennial crop: Fusarium crown and root rot of asparagus is related to changes in soil microbial community structure,” Plant Soil, vol. 268, no. 1, pp. 75–87, 2005, doi: 10.1007/s11104-004-0228-1.

S. Basharat, S. Mazhar, R. Yasmeen, and W. Hamid, “Evaluation of Microbial Contamination via Wastewater Collected from Different Oil Industries and its Treatment Using Various Coagulants,” Int. J. Innov. Sci. Technol., vol. 4, no. 2, pp. 392–403, 2022, [Online]. Available:




How to Cite

Hussain , K. ., Waseem, M., Mumtaz , I., & Riaz, D. S. (2022). Molecular characterization of Deciphering Fungal Community structure in Zea mays L. and Triticum Aestivum L. International Journal of Innovations in Science & Technology, 4(3), 727–737. Retrieved from