Evaluation of Microbial Contamination via Wastewater Collected from Different Oil Industries and its Treatment Using Various Coagulants


  • Roheela yasmeen Lahore Garrison University, Lahore
  • Saman Basharat Department of Biology, Lahore Garrison University, Sector C, DHA Phase VI, Lahore
  • Sumaira Mazhar Department of Biology, Lahore Garrison University, Sector C, DHA Phase VI, Lahore
  • Wajeeha Hamid Department of Biology, Lahore Garrison University, Sector C, DHA Phase VI, Lahore


Wastewater treatment, natural coagulants, chemical coagulants, water pollution


Wastewater from industrial discharged into other water bodies that pose serious risks to human health as well as the environment. The oil and ghee industries are also the main contributors to water pollution along with various other industries. The present study aimed to evaluate microbial load in waste water of oil industries in Lahore and its treatment using chemical and natural coagulants. Water samples were collected from three selected oil and ghee industries in Lahore. Physicochemical properties (Chemical oxygen demand (COD), Biological oxygen demand (BOD), and turbidity) and microbial contamination of water samples were analyzed before and after treatment. It was observed that samples treated with natural coagulants such as orange and banana peel, and date seeds showed a mild reduction in physicochemical parameters. Orange and banana peel coagulants caused a 30% reduction, while date seeds coagulants caused a 60% reduction in physicochemical parameters. A significant decrease in microbial load was noticed by using natural coagulants. However, for the chemical coagulants, it was observed that ferric chloride with alum and Ca+2 cation with bleaching powder caused an extreme reduction in physicochemical indicators and microbial load. While no significant decrease was observed in physicochemical indicators and microbial load when waste water samples were treated with Poly Aluminum chloride (PAC) and alum. It was concluded that chemical coagulants have a better ability to treat waste water as compared to natural coagulants. 

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Edokpayi, J. N., Odiyo, J. O., & Durowoju, O. S., “Impact of wastewater on surface water quality in developing countries: a case study of South Africa” 2017. Water quality, 10: 66561.

Owa, F. D., “Water pollution: sources, effects, control, and management” 2013. Mediterranean journal of social sciences, Vol 4, Issue 8, pp 65-65.

Sharma, P., Tripathi, S., & Chandra, R., “Highly efficient phytoremediation potential of metal and metalloids from the pulp paper industry waste employing Eclipta alba (L) and Alternanthera philoxeroide (L): Biosorption and pollution reduction” 2021. Bioresource Technology, Vol 319, Issue 1, pp 124-147.

Ado, A., Tukur, A. I., Ladan, M., Gumel, S. M., Muhammad, A. A., Habibu, S., & Koki, I. B., “A review on industrial effluents as major sources of water pollution in Nigeria, 2015. Chemistry Journal, Vol 1, Issue 5, pp 159-164.

Galadima, A., Garba, Z. N., Leke, L., Almustapha, M. N., & Adam, I. K., “Domestic water pollution among local communities in Nigeria-causes and consequences” 2011. European journal of scientific research, Vol 52, Issue 4 pp 592-603.

Aniyikaiye, T. E., Oluseyi, T., Odiyo, J. O., & Edokpayi, J. N., “Physico-chemical analysis of wastewater discharge from selected paint industries in Lagos, Nigeria” 2019. International journal of environmental research and public health, Vol 16, Issue 7, pp 12-35.

Boelee, E., Geerling, G., van der Zaan, B., Blauw, A., & Vethaak, A. D., “Water and health: From environmental pressures to integrated responses” 2019. Acta tropica, Vol 19, Issue 31, pp 217-226.

Ang, W.L. and A.W. Mohammad, “State of the art and sustainability of natural coagulants in water and wastewater treatment” 2020. Journal of Cleaner production, Vol 262, Issue 1, pp121267.

Gandiwa, B. I., Moyo, L. B., Ncube, S., Mamvura, T. A., Mguni, L. L., & Hlabangana, N., “Optimisation of using a blend of plant based natural and synthetic coagulants for water treatment: (Moringa Oleifera-Cactus Opuntia-alum blend)” 2020. South African Journal of Chemical Engineering, Vol 34, Issue 1, pp 158-164.

Gautam, S. and G. Saini., “Use of natural coagulants for industrial wastewater treatment” 2020. Global Journal of Environmental Science and Management, Vol 6, Issue 4, pp553-578.

Ilyas, M., Ahmad, W., Khan, H., Yousaf, S., Yasir, M., & Khan, A., “Environmental and health impacts of industrial wastewater effluents in Pakistan: a review” 2019. Reviews on environmental health, Vol 34, Issue 2, PP171-186.

Walter, W.G., “Standard methods for the examination of water and wastewater”, 1961, American Public Health Association.


Ekopai, J. M., Musisi, N. L., Onyuth, H., Gabriela Namara, B., & Sente, C., “Determination of bacterial quality of water in randomly selected swimming pools in Kampala City, Uganda” 2017. New Journal of Science, Vol 4, Issue 1, pp1-7.

An, Y.-J., D.H. Kampbell, & G.P., “Breidenbach, Escherichia coli and total coliforms in water and sediments at lake marinas” 2002. Environmental Pollution, Vol 120, Issue 3, pp 771-778.

Haseena, M., Malik, M. F., Javed, A., Arshad, S., Asif, N., Zulfiqar, S., & Hanif, J., “Water pollution and human health” 2017. Environmental Risk Assessment and Remediation, Vol 1, Issue 3, pp 1-15.

Cristian, O., “Characteristics of the untreated wastewater produced by food industry”, 2017. Analele Universităţii din Oradea, Fascicula: Protecţia Mediului, Vol 15, Issue 1, pp 709-714.

Boutilier, L., Jamieson, R., Gordon, R., Lake, C., & Hart, W., “Adsorption, sedimentation, and inactivation of E. coli within wastewater treatment wetlands” 2009. Water research, 2009. Vol 43, Issue 17, pp4370-4380.

Murtaza, G. & M.H. Zia., “Wastewater production, treatment and use in Pakistan. in Second regional workshop of the project ‘safe use of wastewater in agriculture”, 2012.

Matilainen, A., M. Vepsäläinen, & M. Sillanpää, “Natural organic matter removal by coagulation during drinking water treatment: A review” 2010. Advances in colloid and interface science, Vol 159, Issue 2, pp189-197.

Imran, H., “Wastewater monitoring of pharmaceutical industry: treatment and reuse options” 2005. Electron J Environ Agric Food Chem, Vol 4, Issue 4, pp 994-1004.

Anjaneyulu, Y., N.S. Chary, & D.S.S. Raj., “Decolourization of industrial effluents–available methods and emerging technologies–a review” 2005. Reviews in Environmental Science and Bio/Technology, Vol 4, Issue 4, pp245-273.

Kakoi, B., Kaluli, J. W., Ndiba, P., & Thiong’o, G., “Banana pith as a natural coagulant for polluted river water” 2016. Ecological engineering, Vol 95, Issue 1, pp699-705.

Temesgen, F., Gabbiye, N., & Sahu, O., “Biosorption of reactive red dye (RRD) on activated surface of banana and orange peels: economical alternative for textile effluent” 2018. Surfaces and interfaces, Vol 12, Issue 1, pp151-159.

Al Khusaibi, T. M., Dumaran, J. J., Devi, M. G., Rao, L. N., & Feroz, S, “Treatment of dairy wastewater using orange and banana peels” 2015. Journal of Chemical and Pharmaceutical Research, Vol 7, Issue 4, pp1385-1391.

Kuhiyop, E., D. Adie, & U. Abubakar, “Application of Mangifera indica (mango) and Phoenix dactylifera (dates) seeds powders as coagulants in wastewater treatment” 2020. Nigerian Journal of Technology, Vol 39, Issue 1, pp269-277.

Rambabu, K., Bharath, G., Banat, F., & Show, P. L., “Green synthesis of zinc oxide nanoparticles using Phoenix dactylifera waste as bioreductant for effective dye degradation and antibacterial performance in wastewater treatment” 2021. Journal of hazardous materials, Vol 402, Issue 1, pp123-560.

Abdus-Salam, N., Ikudayisi-Ugbe, A. V., & Ugbe, F. A., “Adsorptive removal of methylene blue from synthetic wastewater using date palm seeds, goethite and their composite” 2021. Acta Scientifica Malaysia (ASM), Vol 5, Issue 1, pp27-35.

Nimesha, S., Hewawasam, C., Jayasanka, D. J., Murakami, Y., Araki, N., & Maharjan, N., “Effectiveness of natural coagulants in water and wastewater treatment” 2021. Global Journal of Environmental Science and Management. Vol 8, Issue 1,pp101-116.

Kurniawan, S. B., Abdullah, S. R. S., Imron, M. F., Said, N. S. M., Ismail, N. I., Hasan, H. A., Othman, A.R., & Purwanti, I. F, “Challenges and opportunities of biocoagulant/bioflocculant application for drinking water and wastewater treatment and its potential for sludge recovery” (2020). International Journal of Environmental Research and Public Health, Vol 17, Issue 24, 9312.




How to Cite

yasmeen, R., Basharat, S., Mazhar, S. ., & Hamid, W. (2022). Evaluation of Microbial Contamination via Wastewater Collected from Different Oil Industries and its Treatment Using Various Coagulants. International Journal of Innovations in Science & Technology, 4(2), 392–403. Retrieved from https://journal.50sea.com/index.php/IJIST/article/view/224

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